94 Cellulase producing strains were isoated from soils, composts, rotten woods and straws, and gastric contents and feces of herbivorous animals in various places. Among them, the strain MC-9, MC-10, MC-53 and MC-51 were found to be highly active in the degradation of carboxy methyl cellulose. Their cultural conditions adequate for the cellulase formation and effects of inorganic salts and various organic substances added to the wheat bran media were investigated.
The results obtained are as follows;
1. Optimum conditions for the cellulase formation were
MC-9: pH 5.5, temp. 35℃, incubation time 5 days,
MC-10: pH 5.5-6.0, temp. 30℃, incubation time 5 days,
MC-53: pH 3.5, temp. 30℃, incubation time 5 days,
MC-61: pH 3.5-4.0, temp. 30-35℃, incubation time 5 days.
2. Their cellulase activity in their optimum conditions were
MC-9: CMC-LP(liquefying power) 87.7%, CMC-SP(saccharifying power) 3.20 glucose ㎎./gm. of the cultures/min.,
MC-10: CMC-LP 82.9%, CMC-SP 2.48 glucose ㎎./gm. of the cultures/min.,
MC-53: CMC-LP 72.4%, CMC-SP 1.76 glucose ㎎./gm. of the cultures/min.,
MC-61: CMC-LP 87.1% CMC-SP 2.68 glucose ㎎./gm. of the cultures/min.
3. Additions of inorganic salts to the wheat bran media were not significant for the cellulase formation, but additions of soybean film and orange-peel pomace promoted the CMC-liquefying power 3 to 5 percent in wheat bran cultures of the strains.
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